Determination of Veterinary Pharmaceuticals Residues in Wastewater of Poultry by HPLC-DAD

Creative Commons License

Erdemoğlu S., Özer Bahşi F., Bahşi İ.

18th International Chromatography Congress, İstanbul, Turkey, 15 - 18 November 2018, vol.1, no.1, pp.40-42

  • Publication Type: Conference Paper / Full Text
  • Volume: 1
  • City: İstanbul
  • Country: Turkey
  • Page Numbers: pp.40-42
  • Inonu University Affiliated: Yes


Veterinary drugs are used inevitably in animal breeding for therapeutic or disease-preventive reasons in parallel with promotinggrowth of livestock, poultry and fish farming. In recent decades, especially veterinary antibiotics have been extensively used in animal farming. Antibiotic residues in edible animal tissues and the emergence of resistant bacteria have received much attention. Large amounts of administrated antibiotics excreted by animals in unchanged form or as metabolites are polluting the aquatic environment through several ways, including leaching and runoff of the manure of animals the overland flow of slurry and the drainage of wastewater from animal farms into rivers, lakes, and agricultural lands directly or after treatment of incomplete removal. As a result, antibiotics excreted by animals affect organisms existed in the environment and lead to the occurrence of antibiotic resistance in bacteria which can threaten humans’ health indirectly. To mitigate the environmental and health risk of antibiotics, their use as animal growth promoters has been banned in EU countries and USA. Therefore the emergent contamination to the aquatic environment has attracted growing public attention and a series of studies have shown that the surface water and groundwater have been contaminated by veterinary [1,2].

In order to monitor antibiotics residues in aquatic environment more efficiently, it is essential to develop an easy and sensitive analytical method capable of simultaneous detection of various antibiotic residues in different water matrices. Several analytical methodologies have been tested for the determination of antibiotics in surface water and wastewaters from ng/L to µg/L. Due to factors such as low analyte concentrations, complex environmental matrixes and diverse physicochemical properties of the antibiotics, their accurate determination remains as a great challenge for environmental studies. The reported methods include liquid-liquid extraction and solid phase extraction (SPE) to pre-concentration and clean-up extracts, and subsequent separation by HPLC, and detection by UV-DAD, fluorescence and mass spectrometry.

This study focuses on determination of antibiotics in wastewater of poultry in Malatya around. Although maximum residue limits of some veterinary antibiotics in animal foodstuff, no regulations have been issued to control the discharge of antibiotics-containing poultry and livestock wastewaters. Since monitoring data of antibiotics residues in the environment around poultry in Malatya have not been reported, as compared with those in EU countries. This paper reports the results our work on multi-residue analysis using HPLC-DAD to determine the concentrations of target antibiotics in wastewater, with a SPE procedure.

Key words: Veterinary drugs, SPE, HPLC-DAD, wastewater