18th International Chromatography Congress, İstanbul, Türkiye, 15 - 18 Kasım 2018, cilt.1, sa.1, ss.40-42
Veterinary
drugs are used inevitably in animal breeding for therapeutic or
disease-preventive reasons in parallel with promotinggrowth
of livestock, poultry and fish farming. In recent decades, especially veterinary
antibiotics have been extensively used in animal farming. Antibiotic residues
in edible animal tissues and the emergence of resistant bacteria have received
much attention. Large amounts of administrated antibiotics excreted by animals
in unchanged form or as metabolites are polluting the aquatic environment
through several ways, including leaching and runoff of the manure of animals
the overland flow of slurry and the drainage of wastewater from animal farms
into rivers, lakes, and agricultural lands directly or after treatment of
incomplete removal. As a result, antibiotics excreted by animals affect
organisms existed in the environment and lead to the occurrence of antibiotic
resistance in bacteria which can threaten humans’ health indirectly. To
mitigate the environmental and health risk of antibiotics, their use as animal
growth promoters has been banned in EU countries and USA. Therefore the
emergent contamination to the aquatic environment has attracted growing public
attention and a series of studies have shown that the surface water and
groundwater have been contaminated by veterinary [1,2].
In
order to monitor antibiotics residues in aquatic environment more efficiently,
it is essential to develop an easy and sensitive analytical method capable of
simultaneous detection of various antibiotic residues in different water
matrices. Several analytical methodologies have been tested for the
determination of antibiotics in surface water and wastewaters from ng/L to
µg/L. Due to factors such as low analyte concentrations, complex environmental
matrixes and diverse physicochemical properties of the antibiotics, their
accurate determination remains as a great challenge for environmental studies.
The reported methods include liquid-liquid extraction and solid phase
extraction (SPE) to pre-concentration and clean-up extracts, and subsequent
separation by HPLC, and detection by UV-DAD, fluorescence and mass
spectrometry.
This
study focuses on determination of antibiotics in wastewater of poultry in Malatya
around. Although maximum residue limits of some veterinary antibiotics in
animal foodstuff, no regulations have been issued to control the discharge of
antibiotics-containing poultry and livestock wastewaters. Since monitoring data
of antibiotics residues in the environment around poultry in Malatya have not
been reported, as compared with those in EU countries. This paper reports the
results our work on multi-residue analysis using HPLC-DAD to determine the
concentrations of target antibiotics in wastewater, with a SPE procedure.
Key words: Veterinary drugs, SPE, HPLC-DAD, wastewater