Research Information System
BIOCATALYSIS AND BIOTRANSFORMATION, no.42, pp.194-211, 2024 (SCI-Expanded)
High cost and low operational stability are the most important challenges limiting the possibleuse of laccase in the removal of textile dyes. To overcome these challenges, in this study, poly-vinylpyrrolidone (PVP)-coated magnetic nanoparticles (MNPs) were produced and characterized.To our knowledge, this is the first study to explore the feasibility of immobilizing a Trametes tro-gii laccase enzyme on Fe3O4/PVP MNPs. The characterization of samples and the successfulimmobilization of laccase were verified by characterization methods. Besides, the biochemicalproperties and stability of the Fe3O4/PVP/Lac were evaluated in terms of optimum pH, optimumtemperature, thermostability, thermodynamic and kinetic parameters, storage stability, oper-ational stability, and decolorization efficiency of two different textile dyes. The optimum activ-ities were recorded at pH 2.5 C and 30 C. The Fe3O4/PVP/Lac displayed remarkable thermalstability and activation energy for denaturation, enthalpy, Gibbs free energy, and entropy resultsconfirmed the enhanced stability of Fe3O4/PVP/Lac against high temperatures. Meanwhile, theFe3O4/PVP/Lac retained about 58% of its original activity after seven consecutive reuses, while itretained up to 25% of its original activity after 28 d of storage at room temperature. Km andVmax for the Fe3O4/PVP/Lac were calculated to be 126 mM and 211 mmol/min, respectively.Finally, after 8 and 6 cycles of repeated use, the Fe3O4/PVP/Lac still decolorized 32.34% and32.23% of Remazol Brilliant Blue R (RBBR) and Indigo Carmine (IC), respectively. As envisioned,this study suggests a promising way to solve the problems of high price and poor operationalstability of the enzyme during biocatalytic decolorization of textile dyes in wastewaters.