FRONTIERS IN NUTRITION, cilt.12, ss.1-14, 2026 (SCI-Expanded, Scopus)
This study investigates the application of thermosonication (TS) to improve the
functional properties of roka (Eruca vesicaria subsp. sativia) water. Processing
parameters, including time (8–16 min), amplitude (60–100%), and temperature
(40–60 °C), were optimised using a comparative approach combining the response
surface method (RSM) and support vector regression (SVR). The total phenolic
content (TPC) increased to 86.04 mg GAE/100 mL with TS, representing an 8.1%
rise compared to the control group and an 18.3% increase over pasteurization.
Likewise, the total chlorophyll level reached 16.98 mmol TE/L from 9.67 g/100 mL,
and β-carotene rose to 24.90 mg/100 mL (p < 0.05). Pasteurization caused losses
of 15–30% in these components. In the phenolic profile, significant increases
were observed in chlorogenic acid (42.05 μg/mL), caffeic acid (15.66 μg/mL),
and quercetin (4.28 μg/mL). A total of 31 compounds were identified in aroma
analysis; with TS treatment, levels of 3-Hexen-1-ol (15.70 μg/kg) and 1-hexanol
(2.01 μg/kg) were preserved or increased. In in vitro digestion tests, the TS group
demonstrated the highest bioavailability, even during the intestinal phase. For
example, RSM demonstrated high compliance coefficients (R2 = 0.99), while SVR
showed strong predictive performance (CV R2 = 0.84), particularly for FRAP. Overall,
the results suggest that thermosonication is an innovative method for protecting
and enhancing bioactive compounds in rocket juice.