Improving nutritional quality, aroma profile and bioactive retention of rocket juice via thermosonication: a support vector regression-based optimization


Levent O., Şimşek M. A., Yıkmış S., Demirel S., Türkol M., Demirok N. T., ...Daha Fazla

FRONTIERS IN NUTRITION, cilt.12, ss.1-14, 2026 (SCI-Expanded, Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 12
  • Basım Tarihi: 2026
  • Doi Numarası: 10.3389/fnut.2025.1721498
  • Dergi Adı: FRONTIERS IN NUTRITION
  • Derginin Tarandığı İndeksler: Scopus, Science Citation Index Expanded (SCI-EXPANDED), Directory of Open Access Journals
  • Sayfa Sayıları: ss.1-14
  • İnönü Üniversitesi Adresli: Evet

Özet

This study investigates the application of thermosonication (TS) to improve the

functional properties of roka (Eruca vesicaria subsp. sativia) water. Processing

parameters, including time (8–16 min), amplitude (60–100%), and temperature

(40–60 °C), were optimised using a comparative approach combining the response

surface method (RSM) and support vector regression (SVR). The total phenolic

content (TPC) increased to 86.04 mg GAE/100 mL with TS, representing an 8.1%

rise compared to the control group and an 18.3% increase over pasteurization.

Likewise, the total chlorophyll level reached 16.98 mmol TE/L from 9.67 g/100 mL,

and β-carotene rose to 24.90 mg/100 mL (p < 0.05). Pasteurization caused losses

of 15–30% in these components. In the phenolic profile, significant increases

were observed in chlorogenic acid (42.05 μg/mL), caffeic acid (15.66 μg/mL),

and quercetin (4.28 μg/mL). A total of 31 compounds were identified in aroma

analysis; with TS treatment, levels of 3-Hexen-1-ol (15.70 μg/kg) and 1-hexanol

(2.01 μg/kg) were preserved or increased. In in vitro digestion tests, the TS group

demonstrated the highest bioavailability, even during the intestinal phase. For

example, RSM demonstrated high compliance coefficients (R2 = 0.99), while SVR

showed strong predictive performance (CV R2 = 0.84), particularly for FRAP. Overall,

the results suggest that thermosonication is an innovative method for protecting

and enhancing bioactive compounds in rocket juice.