Akademik Veri Yönetim Sistemi
5th Eurasia Biochemical Approaches & Technologies (EBAT), Antalya, Türkiye, 2 - 05 Kasım 2023, ss.1
SARS-CoV-2 is a crucial causative agent
of pandemic and early detection of it will always become significant. The most
commonly used diagnostic method is molecular detection by Real-time reverse
transcription polymerase chain reaction (rRT-PCR). In the meantime, antibody
(Ab) detection developed after the viral infection in the patients is another
common method. However, false negative results and advanced devices for
analysis are the main disadvantages of rRT-PCR, while the late production of
Abs about 7-10 days during the infection and cross reactivity of Abs are
drawbacks of the second method. Therefore, rapid antigenic recognition of
SARS-CoV-2 is still being developed by using aptamers, antibodies and viral
surface proteins such as Spike (S) or nucleocapsid (N) protein. Dot blot assay
is one of the rapid assays used in this purpose.1,2 Thus, we
designed the dot blot assay for the rapid detection of SARS-CoV-2 with naked
eye, in this research. For that purpose, gold nanoparticles were conjugated
with aptamer as a capture reagent and receptor binding domain of S protein
(sRBD) was used as target using nitrocellulose membrane. Results showed that
the recognition of SARS-CoV-2 as a synthetic target was accomplished on the
membrane and it was decided to prepare the detection platform for the clinical
samples using dot blot assay.