The Effects of Body Mass Index on Second-Trimester Amniotic Fluid Cytokine and Matrix Metalloproteinase Levels


MELEKOĞLU R. , ÇİFTÇİ O. , Eraslan S., BAŞAK TÜRKMEN N. , Celik E.

GYNECOLOGIC AND OBSTETRIC INVESTIGATION, vol.83, no.1, pp.70-75, 2018 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 83 Issue: 1
  • Publication Date: 2018
  • Doi Number: 10.1159/000455192
  • Title of Journal : GYNECOLOGIC AND OBSTETRIC INVESTIGATION
  • Page Numbers: pp.70-75
  • Keywords: Body mass index, Cytokines, Interleukins, Matrix metalloproteinases, Obesity, Pregnancy, OBESE MOTHERS, INFLAMMATION, PREGNANCY, PLACENTA, FETAL

Abstract

Aim: The aim of this study was to determine the effects of obesity on amniotic fluid (AF) inflammatory markers in second-trimester AF, testing the hypothesis that there is a relationship between maternal body mass index (BMI) and fetal inflammatory exposure. Methods: AF was obtained from 84 singleton pregnant women undergoing elective amniocentesis for karyotype analysis at 16-24 weeks of gestation between April 2014 and May 2016. The cell-free AF was used to analyze interleukin (IL)-1 beta and IL-6, and matrix metalloproteinase (MMP)-1, MMP-6, and MMP-13. Results: IL-1 beta levels were significantly higher in class II-III obese patients than in class I obese, overweight, and normal weight patients (14.68 +/- 1.37 vs. 13.34 +/- 1.86 vs. 13.00 +/- 2.22 vs. 10.78 +/- 1.92, respectively; p < 0.05). IL-6 levels were lowest in the normal weight group and highest in class II-III obese patients. MMP-1, MMP-6, and MMP-13 levels were also significantly higher in class II-III obese patients than in the other groups. Conclusion: This study demonstrated that the fetuses of class II-III obese women are exposed in utero to higher cytokine and MMP levels than fetuses of lean women. Modification of current cutoff levels of intra-amniotic cytokines and MMPs according to the BMI could improve the accuracy of the prenatal diagnosis of intra-amniotic infection and inflammation. (C) 2017 S. Karger AG, Basel