Research Information System
ACTA PHYSIOLOGICA, vol.724, pp.49, 2022 (SCI-Expanded)
AIM: Prangos ferulacea (PF) is a plant which is widely grown and consumed in the world. The aim of the study is to examine whether the PF plant has a hepatotoxic effect, to evaluate the relationship of this effect with ER stress and the effect of JWH-133 on this toxicity.METHODS: Wistar albino male rats aged 8-10 weeks were used in the study (n=37). PF hydroalcoholic extract (PFE) was obtained by us by appropriate methods in our laboratory. The animals were splited into 4 groups as rats in the control group were group-1, rats injected with PFE(250mg/kg/day) were group-2, PFE(250mg/kg/day) and cannabinoid type2 receptor agonist (JWH-133)(1mg/kg/day) injected rats group-3, JWH-133(1mg/kg/day) injected rats group-4. Levels of homogenates obtained from half of the liver tissue, CHOP, GRP78, ATF4, calpain and HSP72 were evaluated by ELISA method. Tissues in the other half of the liver were examined histologically by hematoxylin-eosin staining and immunohistochemically by Caspase-3 staining. Results were calculated by appropriate statistical methods and p<0.05 was considered significant.RESULTS: At the end of our study, the body weights of animals injected with PFE were found to be significantly lower(p<0.05). There was no statistically significant difference between groups in some ER stress markers (CHOP, GRP78, ATF4) and HSP72. Calpain levels were significantly higher in PFE groups compared to the other groups(p<0.05). Immunohistochemical and histo-pathological evaluations showed that caspase-3 was positive in the PFE groups and there was a significant increase in tissue damage (p<0.05). On the other hand, animals treated with PFE and JWH-133 showed a significant improvement in tissue damage(p<0.05).CONCLUSION: In our study, it was observed that Prangos Ferulacea plant increased liver damage independently of ER stress in terms of immunohistochemistry and histology, while JWH-133 reduced this damage.