Akademik Veri Yönetim Sistemi
4. EBAT, Antalya, Türkiye, 3 - 06 Kasım 2022, ss.16-17
The Coronavirus disease 2019 (COVID-19) pandemic
continues to spread across the world. Thus, there is an urgent need for rapid,
simple and accurate tests to diagnose severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2) infection.1 The mostly used and reliable method is the RT PCR
recommended by the WHO. Since the N gene region is the most conserved and
specific, it is important molecular
diagnosis. Compared to the RT PCR method, rapid diagnostic kits have been used
as an alternative method because they are easy to use and the short time to get
results. 2 For this purpose, our study aimed to determine one region
of N gene specific to SARS-CoV-2 with the Lateral Flow Test
(LFT) platform. The LFT platform is designed on the basis of hybridization with
sandwich model based on Gold nanoparticles (AuNPs). The designed LFT was
especially designed for the conserved region of the N gene. AuNPs were
conjugated with oligonucleotides complementary to the N gene. Sequences to
hybridize to this conjugation product were plotted as test line on the
nitrocellulose membrane. The sample containing the target sequence hybridizes
with the sequences in the test and control line according to the principle of
capillary flow when applied to the sample pad. A red color formation from of AuNPs
can be seen by naked eye. According to the research findings designed LFT
recognized the SARS-CoV-2 N gene region specifically and turned out to be an
alternative to molecular diagnosis