Hydrodistilled volatile oil from the aerial parts of Achillea cretica L. (Asteraceae) was analysed by a combination of GC and GC/MS. Seventy-six components were identified, constituting 86.4% of the oil. The main constituents of the essential oil were caryophylladienol-II (13.4%), beta-maaliene (6.1%), neo-intermedeol (6.0%), carvone (4.9%), spathulenol (4.5%), palmitic acid (3.3%) and selina-3,11-dien-6 alpha-ol (3.2%). The antimicrobial activity was evaluated by the broth-dilution method on nine microbial strains and showed to be quite strong against the Gram-positive bacteria Staphylococcus aureus and Bacillus cereus. The antibacterial properties of A. cretica justify its use in traditional medicine for the treatment of wounds, contaminated through bacterial infections.