Synthesis, structural characterization, and in vitro anti-cancer activities of new phenylacrylonitrile derivatives

Ozen F., TEKİN S., Koran K., SANDAL S., Gorgulu A. O.

APPLIED BIOLOGICAL CHEMISTRY, cilt.59, sa.2, ss.239-248, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 59 Sayı: 2
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1007/s13765-016-0163-x
  • Dergi Adı: APPLIED BIOLOGICAL CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.239-248
  • Anahtar Kelimeler: Anti-tumor activity, Breast cancer, MCF-7, NMR-spectroscopy phenylacrylonitrile, ADJUVANT TAMOXIFEN THERAPY, SPECTRUM CYTOTOXIC AGENTS, BREAST-CANCER, ANALOGS, CELLS
  • İnönü Üniversitesi Adresli: Evet

Özet

The present study was designed to both synthesize phenylacrylonitrile compounds (1a-k) and their anti-tumor activities on human breast cancer cell line (MCF-7) were determined. The structures of all the compounds were defined by melting point, elemental analysis, FT-IR, H-1, C-13, C-13-APT, and HETCOR-NMR spectroscopy. Anti-tumor activities of these compounds on cell viability were evaluated using 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide assay against MCF-7. The MCF-7 cell lines were treated with 1, 5, 25, 50, and 100 mu M concentrations of phenylacrylonitrile compounds for 24 h. At the end of the experiments, 1a, 1b, 1c, 1g, and 1h compounds reduced cell viability (p < 0.01). Additionally, the anti-cancer activities of these compounds on MCF-7 were investigated by comparing IC50 values. In conclusion, while some of the synthesized phenylacrylonitrile compounds (1a, 1b, 1c, 1g, and 1h) have antitumor activity, other phenylacrylonitrile compounds (1d, 1e, 1f, 1k, and 1h) have no effect on human breast cell lines.