Production of methionine gamma- lyase in recombinant Citrobacter freundii bearing the hemoglobin gene


KAHRAMAN H. , Aytan E., Kurt A. G.

BMB REPORTS, cilt.44, ss.590-594, 2011 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 44 Konu: 9
  • Basım Tarihi: 2011
  • Doi Numarası: 10.5483/bmbrep.2011.44.9.590
  • Dergi Adı: BMB REPORTS
  • Sayfa Sayıları: ss.590-594

Özet

The production of antileukemic enzyme methionine gamma-lyase (MGL) in distinctly related bacteria, Citrobacter freundii and in their recombinants expressing the Vitresocilla hemoglobin (VHb) has been studied. This study concerns the potential of Citrobacter freundii expressing the Vitreoscilla hemoglobin gene (vgb) for the methionine gamma- liyase production. Methionine gamma- liyase production by Citrobacter freundii and its vgb(-) and vgb(+) bearing recombinant strain was studied in shake-flasks under 200 rpm agitation, culture medium and 30 degrees C in a time-course manner. The vgb(+) and especially the carbon type had a dramatic effect on methionine gamma- liyase production. The vgb(+) strain of C. freundii had about 2-fold and 3.1-fold higher levels of MGL than the host and vgb(-) strain, respectively. [BMB reports 2011; 44(9): 590-594]