Research Information System
21th International Medicine and Heath Sciences Researches Congress, Ankara, Turkey, 6 - 07 March 2026, pp.93-94, (Summary Text)
Santolina chamaecyparissus L. (cotton lavender) is a perennial aromatic shrub of the Asteraceae family, native to the Mediterranean region, including Türkiye. Known for its rich secondary metabolites and diverse biological activities, this study investigates the relatively understudied cytotoxic potential of this species for cancer therapy. This study aimed to evaluate the cytotoxic potential of Santolina chamaecyparissus L. methanol extract against A549 (lung cancer) and Hep3B (hepatocellular carcinoma) cell lines. The plant material was obtained from the Medical and Aromatic Plants Garden of Inonu University Faculty of Pharmacy (Malatya, Türkiye). The aerial parts were macerated in methanol (3x24 h, at room temperature), resulting in a 14.4% yield of the extract. Cytotoxicity was assessed using the MTS assay on A549 and HEP3B cell lines. The cells were treated with five concentrations of the extract (10, 25, 50, 75, and 100 μg/mL) for 24 hours. All experiments were conducted in triplicate. The extract exhibited dose-dependent cytotoxic effects on both cancer cell lines. After 24 hours of treatment, the highest concentration (100 μg/mL) reduced cell viability to 20.7% in Hep3B cells and 4.2% in A549 cells. At the end of 24 hours, IC50 values were calculated as 46.06 μg/mL for the Hep3B line and 44.26 μg/mL for the A549 line. In conclusion, the methanol extract of S. chamaecyparissus was found to exhibit promising anticancer potential against lung and liver cancer cell lines. These findings support further investigation into the specific bioactive compounds and molecular mechanisms responsible for its cytotoxic activity.