In this study, four textile dyes, namely Astrazon Yellow, Red, Blue, and Black, were tested for their genotoxic effects in the wing cells of Drosophila melanogaster. Two crosses were used, the standard cross (ST) and the improved high-bioactivation cross (HB), the latter being characterized by increased sensitivity to the genotoxic effects of promutagens and procarcinogens. Three-day-old larvae were exposed to different concentrations of dyes. Commonly known mutagens were applied as positive controls. All concentrations of textile dyes, ethyl methanesulfonate ( EMS), and urethane caused a decrease in survival proportional to concentration used. EMS and urethane caused an increase in the number of all types of spots in both standard and high-bioactivation crosses. Compared to ST crosses, the number of induced spots in the HB cross treated with urethane was considerably high. Treatment of the standard and the high-bioactivation crosses with textile dyes gave positive results, apparent from increase in the frequency of the small single spots. Yellow and red dyes also increased the number of large single spots in both crosses, whereas the twin spots were positive only at the highest dose of yellow dye. All these results indicate that D. melanogaster wing spot test can be recommended as a suitable in vivo test for the determination of genotoxicity of textile dyes.