Caffeic Acid Based Polyurethane Membrane Modified Screen-Printed Electrodes and Their Application for the Simultaneous Determination of Melatonin and Serotonin

Erel E., Tunç M. G. K., KÖYTEPE S., ATEŞ B., YILMAZ İ.

Analytical Letters, cilt.58, sa.15, ss.2551-2568, 2025 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 58 Sayı: 15
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1080/00032719.2024.2420907
  • Dergi Adı: Analytical Letters
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Food Science & Technology Abstracts, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.2551-2568
  • Anahtar Kelimeler: Caffeic acid, differential pulse voltammetry (DPV), electrochemical analysis, melatonin, polyurethane, serotonin, voltammetric sensor
  • İnönü Üniversitesi Adresli: Evet

Özet

Caffeic acid (CA) based polyurethane (PU) films were synthesized and characterized for the simultaneous determination of melatonin (MT) and serotonin (5-HT). The CA based PUs were synthesized using a polyol mixture of 4,4’-diisocyanodiphenylmethane (MPI), polyethylene glycol-200 (PEG), and CA. The PEG–CA monomer units in the polyol were varied in molar ratios of 99:1, 97:3, 95:5, and 90:10. The synthesized PUs were characterized by FTIR spectroscopy and thermal analysis. These CA based PU films were coated onto screen-printed carbon electrodes (SPCEs) with varying thicknesses and used for the simultaneous determination of MT and 5-HT. Electrochemical responses of MT and 5-HT were evaluated using differential pulse voltammetry. The limit of detection and correlation coefficient for MT were 280 µM and 0.9797, respectively, while for 5-HT, the detection was 150 µM with a correlation coefficient of 0.9690. The precision, expressed as the relative standard deviation was 2.633% for MT and 4.668% for 5-HT. These findings suggest that CA based PU-modified electrodes hold potential in medical and biomedical applications.