Wheat (Triticum spp.) is highly susceptible to the root lesion nematode, Pratylenchus thornei, which causes excessive amounts of crop losses each year. In this research, we investigated the cumulative effect of the biocontrol agent Purpureocillium lilacinum (syn: Paecilonryces Ilk:citrus) against P. !hornet. Three doses of P lilacinum (10(6), 10(7) and 10(8) conidia cultures mL(-1)) with one dose of 400 P. !hornet individuals (adults and juveniles) mL(-1) were applied in 100 cm(3) soil under greenhouse conditions. The number of nematodes in the soil and root in addition to total nematode in soil+root were determined. Moreover, different plant parameters such as the plant height, plant fresh and dry weight, root fresh and dry weight were evaluated. Applications with the higher dose of bio-agents (100 cm(3) P thornet infested soil with 10(8) conidia culture of P. lilacinum mL(-1)) exhibited maximal enhancement in dry and fresh weight of shoot and reduced P. thornei population. As a consequence. P Madman individually was highly effective in enhancing different plant parameters and suppressing P. thornei reproduction. Overall, present findings suggest that the exploitation of the biocontrol agent P. lilacinum could be helpful for effective management of the root lesion nematode P. thornei.