The research interest in fetal heart rate (FHR) monitoring dates back to the 1960s, and the breakthrough on fetal surveillance has been seen during the 1990s with computerized systems. Notwithstanding the general use of cardiotocography (CTG) in fetal monitoring, the assessment of fetal well-being exhibits a significant inter-and even intra-observer variability. Computerized CTG analysis has seen as the most promising way to tackle of the main shortcomings of visual CTG assessment. In this study, a novel software developed for research purposes is introduced. The software named as CTG Open Access Software (CTG-OAS) characterizes FHR signals by using comprehensive features obtained from different fields such as morphological, linear, nonlinear, time-frequency, discrete wavelet transform, and image-based time-frequency domains. The software also covers the main procedures which are necessary for the context of machine learning. More specifically, CTG-OAS presents several tools for performing the preprocessing, feature extraction, feature selection, and classification processes. The proposed software was practiced on CTU-UHB database with 552 raw CTG samples. In addition, a case study with Support Vector Machine classifier was performed in the study via CTG-OAS. According to experimental results, statistical parameters were obtained as accuracy equal to 87.97%, sensitivity equal to 89.04%, specificity equal to 81.36% and, quality index equal to 85.11%.