Laccase production by newly isolated white rot fungus Funalia trogii: Effect of immobilization matrix on laccase production

BİRHANLI E., ERDOĞAN S., YEŞİLADA Ö., ÖNAL Y.

BIOCHEMICAL ENGINEERING JOURNAL, cilt.71, ss.134-139, 2013 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 71
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1016/j.bej.2012.12.002
  • Dergi Adı: BIOCHEMICAL ENGINEERING JOURNAL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.134-139
  • Anahtar Kelimeler: Activated carbon, Enzymes, Enzyme production, Fermentation, Filamentous fungi, Laccase, ENHANCED PRODUCTION, ACTIVATED CARBON, TEXTILE DYES, WASTE-WATER, PLEUROTUS-OSTREATUS, TRAMETES-PUBESCENS, DECOLORIZATION, PELLETS, FERMENTATION
  • İnönü Üniversitesi Adresli: Evet

Özet

The laccase production performance of newly isolated Funalia trogii (F. trogii) immobilized on low-cost activated adsorbents prepared from apricot stone was investigated in inexpensive molasses medium and compared with free pellets. The adsorbents were apricot stone-based activated carbon (AC), Cu-impregnated apricot stone-based activated carbon (Cu-AC) and Fe-impregnated apricot stone-based activated carbon (Fe-AC). Cu-AC and Fe-AC were prepared by chemical activation. The values of BET surface area (S-BET), total pore volume (V-t), micropore surface area (S-mic) and micropore volume (V-mic) which show the pore properties of the activated carbons were 133 m(2) g(-1), 0.105 cm(3) g(-1), 108 m(2) g(-1) and 0.056 cm(3) g(-1) for Cu-AC and 145 m(2) g(-1), 0.112 cm(3) g(-1), 117 m(2) g(-1) and 0.061 cm(3) g(-1) for Fe-AC, respectively. F. trogii attached well on all of these adsorbents. However, the fungus immobilized on Cu-AC produced much higher levels of laccase than the others. The laccase activity obtained in 5 g L-1 molasses media after first cycle and mean laccase activity obtained after three cycles with fungus immobilized on Cu-AC were 29.23 U mL(-1) and 27.04 U mL(-1), respectively. These activities were only 0.25 and 0.39 U mL(-1) with free pellets. The crude laccase from immobilized culture could also decolorize the textile dyes. (c) 2012 Elsevier B.V. All rights reserved.