In this study, we evaluated the effects of fertilizers of different origin on broccoli yield and some characteristics. For this purpose, 9 different fertilizers were used. The different fertilizers used in the study were; NT; Control (No Treatment), FM; Farm Manure (approximate 2-4% N) CF; Chemical Fertilizer (46% N, 46% P2O5, 51% K2SO4), HA; Humic Acid, AA; Amino Acid, HFA; Humic and Fulvic Acid, ALG; Microalgea, ART; Arthrobacter sp., BAS; Bacillus subtilis strain QST 713. During the vegetation period, the plant growth parameters were measured twice in the growth season. The first measurement was performed after 40 days of planting, while the second was done after 70 days. Two plants were harvested from each replication in order to determine the plant growth parameters of shoot fresh weight, root fresh weight, plant height, root length, shoot and root dry weight and the number of the leaves. At the end of the experiment, mineral contents of leaves, total yield and ascorbic acid contents of broccoli heads were determined. As a result, it was determined that some organic fertilizers and biofertilizers increased the yield, various plant growth parameters, nutrient uptake of broccoli and ascorbic acid contents of broccoli heads at a significant level.