Protective effects of ebselen against iron-induced cardiotoxicity in rats.

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Karakuş A., Zeren C., Sefil F., Gökçe H., Motor S., Çelik M. M.

Healthmed, cilt.7, sa.5, ss.1424-1430, 2013 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 7 Sayı: 5
  • Basım Tarihi: 2013
  • Dergi Adı: Healthmed
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), CAB Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.1424-1430
  • İnönü Üniversitesi Adresli: Hayır

Özet


Objective: Ebselen, a substance with glutathione peroxidase-like activity, shows antioxidant,
anti-inflammatory, neuroprotective and immunomodulatory effects. The purpose of this study was to find out the effects of ebselen against iron-induced cardiotoxicity in rats.


Material and Method: After obtaining ethical committee approval, fifty-six male Wistar Albino
rats were divided into seven groups. Serum iron, ferritin, lactate dehydrogenase, creatine kinase and creatine kinase-MB (CK-MB) levels were measured biochemically. Iron accumulation in cardiac tissue samples was measured using inductively coupled plasma atomic emission spectrometry (ICP-AES). Comparison of iron accumulation histopathologically performed using Prussian blue stained sections by semi-quantitative method.
Results: Serum LDH levels in ebselen+iron, iron+deferoxamine and iron+deferroksamine+ebselen groups were significantly hihger compared to control group, while significant difference was observed between serum CK-MB level of
iron+deferroksamine+ebselen group and control samples (p<0.001). Serum iron levels in iron+deferoxamine and iron+deferroksamine+ebselen groups were highly significanly hihger than controls, and serum' ferritine levels in iron, ebselen+iron, iron+deferroksamine and iron+deferroksamine+ebselen groups were significantly higher compared to control group (p<0.001). Iron levels of cardiac tissue measured by ICP-AES in iron, iron+deferroksamine and iron+deferroksamine+ebselen applied groups were highly significantly different compared to those in controls (p<0.001), while a relatively less significant difference observed between ebselen+iron group and control (p < 0.05). Iron accumulation was found to be decreased in ebselen and deferroksamine given groups when histopathologically compared to iron
applied group.


Conclusion: In conclusion, obtained findings indicated the efficacy and utility of ebselen
treatment on iron induced cardiotoxicity. In this regard, ebselen might contribute to treatment in P-thalassemia and sickle cell patients, and those in need of frequent blood transfusion.


Key words: Ebselen, heart influences, iron induced toxicity.