The aim of our study is to investigate whether chronic carbon monoxide (CO) exposure has effects on the brains of the people who work as barbecue workers (BWs) for a long time. Twenty males who had been working in the indoor environments of various restaurants (Group I) and 20 healthy males who were matched by age with the other group (Group 2) were included in the study. Laboratory tests were performed in both groups of people. Magnetic Resonance Spectroscopy (MRS) was applied to the people whose blood samples were taken. In Group I, significant correlations were not detected between carboxyhemoglobin (COHb) values and the variables of age, working hours, hemoglobin (Hb), white blood cell (WBC), hematocrit (HCT), platelet (PLT), mean platelet volume (MPV), pH, oxygen saturation (Sat O2) (p>0.05). In terms of Hb, WBC, HCT, PLT, MPV, pH, Sat O2, COHb, N-acetylaspartate/creatine in basal ganglion, choline/creatine in basal ganglion, N-acetylaspartate/creatine in frontal lobe periventricular white matter, choline/creatine in frontal lobe periventricular white matter variables, significant differences between Group I and Group 2 were not determined. (p>0.05). As a result of this study; it is detected that chronic exposure to CO in BWs does not have any effects on brain with respect to MRS imaging method.