It’s believed that determining the teachers’ perception of self-efficacy regarding related to science/chemistry subjects in classes is important. Studies about teachers’ perceived self-efficacy related to a particular chemistry topic need to carry out.. The aim of this study is to develop “Acid-Base Self-Efficacy Perception Scale (ABSPS)’’ and to investigate the level of pre-service science teachers’ self-efficacy perceptions on acid-base topic in terms of various variables (gender, grade, the type of high schools graduated and achievement at General Chemistry II lesson). Survey method was used. The sample of the study consists of 982 pre-service science teachers at eight different state universities. ABSPS, a five-point likert type, consists of two dimensions, developed in the study was used as the data collection tool. The dimensions of ABSPS were determined by exploratory factor analysis and confirmatory factor analysis. Reliability coefficient Cronbach's Alpha of ABSPS was 0.823. The results show that pre-service science teachers’ self-efficacy perceptions on acid-base topic vary in terms of gender, grade and “General Chemistry II” course scores. But, another results show that there were no significant differences pre-service teachers’ self-efficacy perceptions in terms of graduated high school type. Studies which are examining self-efficacy related to Chemistry topics would contribute to the literature in order to develop pre-service teachers’ self-efficacy perception related to chemistry topics.