Effects of deleting mitochondrial antioxidant genes on life span

ÜNLÜ E. S., Koc A.

BIOGERONTOLOGY: MECHANISMS AND INTERVENTIONS, cilt.1100, ss.505-509, 2007 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 1100
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1196/annals.1395.055
  • Dergi Adı: BIOGERONTOLOGY: MECHANISMS AND INTERVENTIONS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.505-509
  • Anahtar Kelimeler: aging, antioxidant genes, mitochondria, ROS, CCS1, OXIDATIVE STRESS, YEAST
  • İnönü Üniversitesi Adresli: Evet

Özet

Reactive oxygen species (ROS) damage biomolecules, accelerate aging, and shorten life span, whereas antioxidant enzymes mitigate these effects. Because mitochondria are a primary site of ROS generation and also a primary target of ROS attack, they have become a major focus area of aging studies. Here, we employed yeast genetics to identify mitochondrial antioxidant genes that are important for replicative life span. In our studies, it was found that among the known mitochondrial antioxidant genes (TTR1, CCD1, SOD1, GL04, TRR2, TRX3, CCS1, SOD2, GRX5, PRX1), deletion of only three genes, SOD1 (Cu, Zn superoxide dismutase), SOD2 (Manganese-containing superoxide dismutase), and CCS1 (Copper chaperone), shortened the life span enormously. The life span decreased 40% for Delta sod1 mutant, 72% for Delta sod2 mutant, and 50% for Delta ccs1 mutant. Deletion of the other genes had little or no effect on life span.