Comparison of chemopreventive effects of Vitamin E plus selenium versus melatonin in 7,12-dimethylbenz(a) anthracene-induced mouse brain damage


BATCIOGLU K. , KARAGOZLER A., OZTURK I., GENC M., BAY A., OZTURK F., ...More

CANCER DETECTION AND PREVENTION, vol.29, no.1, pp.54-58, 2004 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 29 Issue: 1
  • Publication Date: 2004
  • Doi Number: 10.1016/j.cdp.2004.06.006
  • Title of Journal : CANCER DETECTION AND PREVENTION
  • Page Numbers: pp.54-58

Abstract

In this work, the protective effect of Vitamin E plus selenium (Vit E + Se) and melatonin against 7,12-dimethylbenz(a)anthracene (7,12DMBA)-induced changes in superoxide dismutase (SOD), glutathione peroxidase (GSHPx), catalase (CAT) and carbonic anhydrase (CA) activities and malonedialdehyde (MDA) levels of mouse brain were compared. 12-month old mice were divided into four groups each including 10 animals. The first group served as control group. The second group was treated with 7,12-DMBA (20 mg/(kg day)). The third group was treated with 7,12-DMBA and Vitamin E (90 mu g/(individual day)) and selenium (1.8 mu g/(individual day)) simultaneously. The fourth group was treated with 7, 12-DMBA and melatonin (4.2 mg/(kg day)) simultaneously. Treatment continued for 21 days after which the mice were sacrificed and brain homogenates were prepared. 7,12-DMBA treated group exhibited significantly decreased levels of brain SOD, GSHPx, CAT and CA activities and increased MDA levels as compared to control. Vitamin E + Se fully or partially restored enzyme inhibition except for SOD. Lipid peroxidation was also reduced in Vitamin E + Se treated group. Melatonin provided a better protection for SOD, GSHPx and CAT, and a plausible protection for CA activity. Protection against lipid peroxidation measured as MDA in melatonin treated group was appreciable although slightly lesser than the protection provided by Vitamin E + Se. The results imply that Vitamin E + Se and melatonin both provide chemoprevention against 7,12-DMBA-induced oxidative stress in mouse brain. (c) 2004 International Society for Preventive Oncology. Published by Elsevier Ltd. All rights reserved.