An antibody of TNF-alpha did not prevent thioacetamide-induced hepatotoxicity in rats

DEMİREL U., HARPUTLUOĞLU M. M. M., SEÇKİN Y., ÇIRALIK H., Temel I., Ozyalin F., ...Daha Fazla

HUMAN & EXPERIMENTAL TOXICOLOGY, cilt.30, sa.7, ss.560-566, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 30 Sayı: 7
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1177/0960327110374206
  • Dergi Adı: HUMAN & EXPERIMENTAL TOXICOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.560-566
  • Anahtar Kelimeler: infliximab, tumor necrosis factor-alpha, thioacetamide, oxidative stress, liver, NECROSIS-FACTOR-ALPHA, FULMINANT HEPATIC-FAILURE, OXIDATIVE STRESS, IONIZING-RADIATION, SENSITIVE METHOD, TISSUE-INJURY, LIVER, INFLAMMATION, PROTECTS, DAMAGE
  • İnönü Üniversitesi Adresli: Evet

Özet

Tumor necrosis factor (TNF)-alpha antibodies have been shown to reduce liver damage in different models. We investigated the effects of infliximab (a TNF-alpha antibody) on liver damage in thioacetamide (TAA)-induced hepatotoxicity in rats. Group 1 (n = 8) was the control group. In group 2 (n = 8), the TAA group, the rats received 300 mg/kg intraperitoneal (ip) TAA daily for 2 days. In group 3 (n = 8), the TAA + Infliximab (INF) group, infliximab (5 mg/kg ip daily) was administered 48 hours before the first dose of TAA daily for 2 days and was maintained for 4 consecutive days. In group 4 (n = 8), the INF group, the rats received only ip infliximab (5 mg/kg) daily. Livers were excised for histopathological and biochemical tests (thiobarbituric-acid-reactive substances [TBARS], and myeloperoxidase [MPO]). Serum ammonia, aspartate transaminase (AST), alanine transaminase (ALT), TNF-alpha, liver TBARS and MPO levels, and liver necrosis and inflammation scores in the TAA group were significantly higher than in the control and INF groups (all p < 0.01). All parameters except AST were not significantly different between TAA and TAA + INF. In conclusion, our results suggest that oxidative stress plays an important role in TAA-induced hepatotoxicity, and infliximab does not improve oxidative liver damage.