Surgical Removal of Endometrioma Decreases the NF-kB1 (p50/105) and NF-kB p65 (Rel A) Expression in the Eutopic Endometrium During the Implantation Window

Celik O., Celik E., TÜRKÇÜOĞLU I., YILMAZ E., ULAŞ M., Simsek Y., ...Daha Fazla

REPRODUCTIVE SCIENCES, cilt.20, sa.7, ss.762-770, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 20 Sayı: 7
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1177/1933719112466307
  • Dergi Adı: REPRODUCTIVE SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.762-770
  • Anahtar Kelimeler: endometrioma, eutopic endometrium, laparoscopy, NF-kB1, NF-kB p65 (Rel A), FACTOR-KAPPA-B, LAPAROSCOPIC OVARIAN CYSTECTOMY, ALPHA-V-BETA-3 INTEGRIN EXPRESSION, IN-VITRO FERTILIZATION, DECIDUAL PROLACTIN, DNA METHYLATION, SEX STEROIDS, CROSS-TALK, WOMEN, HOXA10
  • İnönü Üniversitesi Adresli: Evet

Özet

We aimed to investigate whether the surgical removal of endometrioma alters the nuclear factor-kappa B1 (NF-kB1; p50/105) and NF-kB p65 (Rel A) expression in the eutopic endometrium of infertile women with endometrioma before and after laparoscopic removal of the ovarian endometrioma during the mid-secretory phase. Infertile women with endometrioma (n = 15) were enrolled. Infertile patients with nonendometriotic ovarian cyst (n = 10) and healthy fertile women (n = 10) were recruited as controls. Endometrial samples were obtained before and 3 months after the laparoscopic cystectomy. The NF-kB1 (p50/105) levels were analyzed by enzyme-linked immunosorbent assay (ELISA) in the endometrium of all groups before and after laparoscopic ovarian cystectomy during implantation window. Expression of NF-kB1 (p50/105) in eutopic endometrium was significantly higher in infertile women with endometrioma compared to nonendometriotic cyst and fertile controls (P < .05). Laparoscopic cystectomy resulted in a significant decrease in NF-kB1 expression in women with endometrioma. The NF-kB p65 (Rel A) immunoreactivity of eutopic endometrium decreased significantly subsequent to the surgical removal of the endometrioma. In conclusion, increased endometrial NF-kB expression may contribute to endometriosis-associated infertility.