Overexpression of yeast YLR177w gene provides tolerance to antifungal drug tioconazole

Koç S., Işık Ö. A., Dündar M., Koç A.

Annals of Medical Research, cilt.29, sa.11, ss.1273-1277, 2022 (Hakemli Dergi)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 29 Sayı: 11
  • Basım Tarihi: 2022
  • Doi Numarası: 10.5455/annalsmedres.2022.06.177
  • Dergi Adı: Annals of Medical Research
  • Derginin Tarandığı İndeksler: TR DİZİN (ULAKBİM), Index Copernicus, Sobiad Atıf Dizini
  • Sayfa Sayıları: ss.1273-1277
  • İnönü Üniversitesi Adresli: Evet

Özet

Aim: To find genes that provide resistance to antifungal drug tioconazole by a genomic

DNA screening approach.

Materials and Methods: Wild-type yeast (BY4741) cells were transformed with a high

copy genomic DNA expression library and transformants that can grow on toxic levels of

Tioconazole were determined. Library plasmids were isolated from the transformants and

sequenced for the identification of potential tioconazole resistance gene(s).

Results: Yeast YLR177w gene was isolated as a potential tioconazole resistance gene.

Its overexpression led cells to tolerate normally toxic levels of tioconazole. Deletion of the

YLR177w gene from the yeast genome made cells more vulnerable to the drug.

Conclusion: Yeast YLR177w gene encodes a putative protein of unknown function. Our

analyses suggest a potential function for the YLR177w gene for tioconazole resistance