Akademik Veri Yönetim Sistemi
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, cilt.160, ss.265-272, 2018 (SCI-Expanded)
This study evaluated the toxic effects and recovery patterns in zebrafish (Danio redo) after exposure to phosalone-based (PBP) and cypermethrin-based (CBP) pesticides. Initially, the 96 h LC50 values of the pesticides were calculated as being 5.35 mu g of active ingredient (AI) L-1 for CBP and 217 mu g AI L-1 for PBP based on measured concentrations. Accordingly, experimental groups were exposed to three sublethal concentrations of pesticides for 96 h, separately, and then zebrafish were transferred to pesticide-free conditions for 10 and 20 days recovery periods. Biochemical markers were assessed including carboxylesterase (CaE), acetylcholinesterase (AChE), glutathione S-transferase (GST), lactate dehydrogenase, glutathione peroxidase, catalase, alanine and aspartate aminotransferase (ALT, AST) activities after the exposure and recovery periods. Also, the pesticide concentrations in test water were quantified by high-performance liquid chromatography (HPLC) analysis. Our results showed that AChE and CaE activities were significantly inhibited and GST was induced by both pesticides after 96 h exposure. For PBP exposure, the decreases for GST induction and CaE inhibition showed a partial recovery in pesticide-free conditions. However, the decreases in AChE activity for CBP exposure and insufficient increases in same enzyme activity for PBP exposure after 20 days in pesticide-free conditions indicated that the projected recovery period was not enough to the recovery of AChE activities and for the improvement of fish health.