Assessment of chronological lifespan dependent molecular damages in yeast lacking mitochondrial antioxidant genes

Demir A. B., Koc A.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, cilt.400, sa.1, ss.106-110, 2010 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 400 Sayı: 1
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1016/j.bbrc.2010.08.019
  • Dergi Adı: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.106-110
  • Anahtar Kelimeler: Chronological aging, Molecular damage, Mitochondria, Antioxidant gene, Oxidative stress, OXIDATIVE-STRESS, SACCHAROMYCES-CEREVISIAE, THIOREDOXIN REDUCTASE, SUPEROXIDE-DISMUTASE, DNA-DAMAGE, OXYGEN, OVEREXPRESSION, INSTABILITY, DYSFUNCTION, SULFOXIDE
  • İnönü Üniversitesi Adresli: Evet

Özet

The free radical theory of aging states that oxidative damage to biomolecules causes aging and that antioxidants neutralize free radicals and thus decelerate aging. Mitochondria produce most of the reactive oxygen species, but at the same time have many antioxidant enzymes providing protection from these oxidants. Expecting that cells without mitochondrial antioxidant genes would accumulate higher levels of oxidative damage and, therefore, will have a shorter lifespan, we analyzed oxidative damages to biomolecules in young and chronologically aged mutants lacking the mitochondrial antioxidant genes: GRX2, CCP1, SOD1 GLO4, TRR2, TRX3, CCS1, SOD2, GRX5, and PRX1. Among these mutants, ccp1 Delta, trx3 Delta, grx5 Delta, prx1 Delta, mutants were sensitive to diamide, and ccs1 Delta and sod2 Delta were sensitive to both diamide and menadione. Most of the mutants were less viable in stationary phase. Chronologically aged cells produced higher amount of superoxide radical and accumulated higher levels of oxidative damages. Even though our results support the findings that old cells harbor higher amount of molecular damages, no significant difference was observed between wild type and mutant cells in terms of their damage content. (C) 2010 Elsevier Inc. All rights reserved.