Roles of ATR1 paralogs YMR279c and YOR378w in boron stress tolerance

Bozdag G. O., Uluisik I., Gulculer G. S., Karakaya H. C., Koc A.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, cilt.409, sa.4, ss.748-751, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 409 Sayı: 4
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1016/j.bbrc.2011.05.080
  • Dergi Adı: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.748-751
  • Anahtar Kelimeler: Boron, Boric acid, Yeast, Stress response, ATR1, Ymr279c, TRANSPORTER, RESISTANCE, EVOLUTION, EXPORTER
  • İnönü Üniversitesi Adresli: Evet

Özet

Boron is a necessary nutrient for plants and animals, however excess of it causes toxicity. Previously, Atr1 and Arabidopsis Bor1 homolog were identified as the boron efflux pump in yeast, which lower the cytosolic boron concentration and help cells to survive in the presence of toxic amount of boron. In this study, we analyzed ATR1 paralogs, YMR279c and YOR378w, to understand whether they participate in boron stress tolerance in yeast. Even though these genes share homology with ATR1, neither their deletion rendered cells boron sensitive nor their expression was significantly upregulated by boron treatment. However, expression of YMR279, but not YOR378w, from the constitutive GAPDH promoter on a high copy plasmid provided remarkable boron resistance by decreasing intracellular boron levels. Thus our results suggest the presence of a third boron exporter, YMR279c, which functions similar to ATR1 and provides boron resistance in yeast. (C) 2011 Elsevier Inc. All rights reserved.