In UV-C, Deinonoccus radiodurans and Vitreoscilla Hemoglobin (vgb) Gene Transferred Recombinants; Effect on SOD, KAT and Carotene Amount

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OZBEY E., ASMA D.

KSU TARIM VE DOGA DERGISI-KSU JOURNAL OF AGRICULTURE AND NATURE, cilt.25, sa.3, ss.476-484, 2022 (ESCI) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 25 Sayı: 3
  • Basım Tarihi: 2022
  • Doi Numarası: 10.18016/ksutarimdoga.vi.916575
  • Dergi Adı: KSU TARIM VE DOGA DERGISI-KSU JOURNAL OF AGRICULTURE AND NATURE
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.476-484
  • Anahtar Kelimeler: UV-C radiation, Antioxidant enzyme, Superoxide dismutase, Catalase, Carotene, BACTERIUM DEINOCOCCUS-RADIODURANS, FREE-RADICALS, MICROCOCCUS-RADIODURANS, SUPEROXIDE-DISMUTASE, TARGETED MUTAGENESIS, OXIDATIVE DAMAGE, DNA, RADIATION, CHEMILUMINESCENCE, EXPRESSION
  • İnönü Üniversitesi Adresli: Evet

Özet

Ultraviolet radiation (UV); creates oxidative stress by causing the formation of reactive oxygen species in biological tissues. Reactive oxygen species induced by UV. their effects and cellular defense mechanisms against them, and antioxidant systems responsible for cleaning reactive oxygen species are the subjects of much research today. In this study, Deinococcus radiodrans which is well known an extremophile for its resistance to many agents and conditions such as high levels of ionizing radiation and UV radiation, drought and chemicals that damage DNA and Vitreoscilla hemoglobin (vgb) gene cloned recombinant with. and vgb recombinant strain as a control were used. The effect of UV-C on the antioxidant defense systems of D. radiodurans (superoxide dismutase, catalase and carotene) was investigated, and in addition, the contribution of the vgb(-) gene, which provides more oxygenated environment to the organism, to the UV resistance of the bacteria, was investigated. Accordingly, when UV-C treated samples of D. radiodurans (vgb(-)) were compared with the control groups, it was determined that the superoxide dismutase and catalase enzyme activities were lower than the wild and the recombinant. carrying the vgb gene. Again, in wild-type bacteria with high carotene. an increase in the amount of carotene was clearly observed due to UV-C application.