Akademik Veri Yönetim Sistemi
Ankara Üniversitesi Eczacılık Fakültesi Dergisi, cilt.47, sa.2, ss.394-407, 2023 (Scopus)
Objective: The aim of the study was to investigate the preventive effects of taurine against 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced organ damage in rats. The environmental toxin TCDD has a high toxicity in animal and human tissues. Taurine is an amino acid found in many organs, with multiple physiological roles including the protection of cells with its antioxidant and anti-inflammatory properties. In this context, our aim in this study was to investigate the potential preventive effect of taurine on oxidative stress and organ damage caused by TCDD in rat liver and kidney tissues. To evaluate these possible effects, we measured the levels of thiobarbituric acid reactive substances (TBARS), and glutathione (GSH), as well as the activity of superoxide dismutase (SOD). In addition, immunohistochemical detection of caspase-3 expression, and the assessment of histopathological changes in tissue samples were performed. Material and Method: Adult male Wistar rats (250-300 g, 12-13 weeks, n = 32) were randomly allocated into four groups (n = 8/group): Control, TCDD, TAU, and TCDD+TAU. TCDD and/or taurine were administered via gavage in doses of 2 μg/kg/week and 200 mg/kg/day, respectively. Result and Discussion: The results showed that TCDD caused oxidative stress in the liver and kidney tissues of rats by decreasing the levels of GSH and SOD activity and increasing the levels of TBARS. Taurine treatment significantly reduced TBARS levels (p<0.05), it significantly increased GSH levels and SOD activity (p<0.05) in the concurrent administration of TCDD and taurine. Taurine also reduced the histopathological changes caused by TCDD-induced oxidative stress in the liver and kidney tissues. Taurine prevented the apoptotic pathway by decreasing cysteine aspartate specific protease-3 (caspase-3). Taurine supplementation helps to regulate oxidative imbalance and reduces histopathological changes caused by TCDD-induced organ damage. This could be a novel approach to avoiding TCDD toxicity.