Metabolomic analysis of endometrial cancer by high-resolution magic angle spinning NMR spectroscopy.

Arda Düz S., Mumcu A., Doğan B., Yılmaz E., İnci Çoşkun E., Sarıdogan E., ...Daha Fazla

Archives of gynecology and obstetrics, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası:
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1007/s00404-022-06587-0
  • Dergi Adı: Archives of gynecology and obstetrics
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CINAHL, EMBASE, MEDLINE
  • Anahtar Kelimeler: Endometrial cancer, Endometrial tissue, H-1-NMR, Metabolomics, CHOLINE PHOSPHOLIPID-METABOLISM, GLUTAMINE-METABOLISM, AMINO-ACIDS, EXPRESSION, DISCOVERY
  • İnönü Üniversitesi Adresli: Evet

Özet

Purpose To analyze endometrial metabolite profiles between patients with endometrial cancer and controls. Methods Seventeen (17) women with endometrium cancer and 18 controls were enrolled in this study. H-1 HR-MAS (High Resolution-Magic Angle Spinning) NMR (Nuclear Magnetic Resonance) spectroscopy data obtained from endometrial tissue samples of patients with endometrial cancer and control group were analyzed with bioinformatics methods. Results Principal component analysis (PCA) and the partial least squares discriminant analysis (PLS-DA) score plots obtained with the multivariate statistical analysis of pre-processed spectral data shows a separation between the samples from patients with endometrial cancer and controls. Analysis results suggest that the levels of lactate, glucose, o-phosphoethanolamine, choline, glycerophosphocholine, phosphocholine, leucine, isoleucine, valine, glutamate, glutamine, n-acetyltyrosine, methionine, taurine, alanine, aspartate and phenylalanine are increased in patients with endometrial cancer compared to the controls. Conclusion The metabolomics signature of patients with endometrial cancer is different from that of benign endometrial tissue.