Akademik Veri Yönetim Sistemi
International Journal of Life Sciences and Biotechnology, cilt.5, sa.3, ss.480-503, 2022 (Hakemli Dergi)
This study aimed to determine the antibacterial and antioxidant activities of the newly synthesized Schiff base and to support the laboratory results with molecular modeling studies. Antibacterial activity of schiff bases was demonstrated using Gram (-) Pseudomonas aeruginosa and Acinetobacter baumanni bacterial strains. Minimum Inhibition Concentration (MIC) values were determined to evaluate their antimicrobial activity against Gram (-) Pseudomonas aeruginosa and Acinetobacter baumanni bacterial strains. In antioxidant experiments, the responses to DPPH and ABTS radicals were calculated at certain concentration ranges and graphs were drawn. For the molecular modeling study, Autodock Vina and Discovery Studio 2020 package programs were used. The observed bacterial inhibition activity varied depending on the clinical isolate and the concentration of the samples tested. The highest inhibition activity was achieved at concentration of 75 μl -100 μl. N,N'-(ethane-1,2-diyl)bis(1-(9Hfluoren-2-yl)methanimine) samples. Molecular docking results show that N,N'-(ethane1,2-diyl)bis(1(9H-fluoren-2-yl) methanimine) binds strongly to the 4ZIY and 4ZHU structures. It has been proven by molecular docking study that the synthesized Schiff base ligand has antibiotic resistance properties. N, N'-(ethane-1,2-diyl)bis(1-(9Hfluoren-2-yl) methanimine) synthesis compound showed moderate activity against A. baumannii and P. aeruginosa strains. It is known that Schiff bases have strong biological activities and antibacterial activity. In this study, the synthesis of Schiff base showed antibacterial and antioxidant activity. In addition, our results were supported by molecular modeling. Our findings can be taken to a higher level with in vivo and in vitro studies.