Voltammetric Determination of 5-Hydroxyindoleacetic Acid at Poly (p-amino benzene sulfonic acid) Modified Sensor

Yilmaz A., Kuyumcu Savan E., Erdoğdu G.

ANALYTICAL & BIOANALYTICAL ELECTROCHEMISTRY, cilt.14, sa.4, ss.418-431, 2022 (ESCI) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 14 Sayı: 4
  • Basım Tarihi: 2022
  • Dergi Adı: ANALYTICAL & BIOANALYTICAL ELECTROCHEMISTRY
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus, Arab World Research Source
  • Sayfa Sayıları: ss.418-431
  • Anahtar Kelimeler: 5-Hydroxyindolacetic acid, Serotonin, p-Amino benzene sulfonic acid, Differential pulse voltammetry, Cyclic voltammetry, GLASSY-CARBON ELECTRODE, PERFORMANCE LIQUID-CHROMATOGRAPHY, ASCORBIC-ACID, ELECTROCHEMICAL DETECTION, CEREBROSPINAL-FLUID, ACETIC-ACID, 5-HYDROXYTRYPTAMINE, NEUROTRANSMITTERS, METABOLITES, SEROTONIN
  • İnönü Üniversitesi Adresli: Evet

Özet

In this study, the quantitative amount of 5-hydroxyindoleacetic acid (5-HIAA) was determined at poly (p-amino benzene sulfonic acid) (p-ABSA) modified glassy carbon electrode. The redox property of 5-HIAA was investigated by cyclic voltammetry. The cyclic voltammograms showed that the redox reaction of 5-HIAA was irreversible. Scan rate study showed that the redox reaction of 5-HIAA was controlled by both diffusion and adsorption on the poly (p-ABSA) modified sensor. Differential pulse voltammetry technique was used for the quantitative analysis of 5-HIAA in phosphate buffer solution at pH 7.00. The linear working range of the calibration graphs was determined as 1x10(-5)-9x10(-5) M (R-2, 0.9912), and the detection limit was determined as 5.3x10(-7) M. Recovery values in the analysis of urine samples were between 99.4% and 103.0%. The results showed that the modified sensor can be applied to the determination of 5-HIAA in the presence of ascorbic acid. The proposed sensor is promising for routine analysis due to its high selectivity, reproducibility, long-term stability, and high recovery values in biological samples.